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Figure 4 | Arthritis Research & Therapy

Figure 4

From: Copper chelation with tetrathiomolybdate suppresses adjuvant-induced arthritis and inflammation-associated cachexia in rats

Figure 4

Immnunostaining for VEGF in synovial tissue in AIA rats. (a),(c), (e) AIA rats treated with deionized water and (b),(d) tetrathiomolybdate (TM). Immunohistochemical staining was performed with the Vecto Stain avidin–biotin peroxidase complex kit (Vector Laboratories, Burlingame, CA, USA). Synovial tissues sections were stained with (a)–(d) mouse anti-VEGF monoclonal IgG antibodies (1:200 dilution, in PBS; Santa Cruz Biotechnology, Santa Cruz California, USA) and (e) a normal mouse IgG (1:200 dilution, in PBS). Positive immunostaining was indicated by brownish deposits. The counterstain was an aqueous solution of hematoxylin. (a), (b) Original magnification × 40; (c)–(e) original magnification × 400. AIA, adjuvant-induced arthritis; ET, endothelial cell; SL, synovial lining cell.

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