Induction of a B-cell-dependent chronic arthritis with glucose-6-phosphate isomerase

Table 1 Correlation between specific IgG-total and accumulative score

Strain	Anti-hG6PI versus score		Anti-CII versus score		n
	Rho	P value	Rho	P value
C3H.NB	0.917*	0.009*	0.793*	0.026*	9
B10.Q	0.402*	0.046*	-0.094	0.614	26
(B10.Q × DBA1)F₁	0.018	0.956	0.139	0.67	10
DBA/1	0.317	0.370	0.033	0.924	10

To investigate correlations between arthritis severity and antibody production the Spearman correlation test was applied. Mice of indicated strains were immunized with 200 μg glucose-6-phosphate isomerase (G6PI) in complete Freund's adjuvant. Blood was drawn at day 40 and analyzed by ELISA for anti-hG6PI and anti-CII total IgG responses, as shown in Fig. 1. The accumulative arthritis score until day 40 was tested for correlation with antibody production using the Spearman rank correlation test. A rho value close to 1 indicates correlation of high ranks for IgG with high ranks for arthritis scores; 0 indicates that there is no correlation between values; and a number close to -1 indicates that high ranks for one variable correlate with low ranks for the other. *Significant positive correlations.

ISSN: 1478-6362