PONDR® predictions compared to experimental structural determinations for various autoantigens. (a) La autoantigen (Swiss-Prot: P05455). The shaded box above the plot (residues 231–325) is the region that Jacks et al.  determined to be ordered via NMR. The empty boxes (residues 214–230 and residues 326–408) are regions determined to be unstructured or disordered. The inset (PDB: 1OWX; La222-334) shows the conformational flexibility of disordered regions at the amino and carboxyl terminii of the La fragment. (b) DNA topoisomerase I (Swiss-Prot: P11387). The structure was determined by X-ray methods for a protein-DNA complex (PDB: 1EJ9) encompassing residues 203–765 of DNA topoisomerase I. Residues 634–713 (empty box) are missing and, therefore, disordered in the structure . The lightly shaded box at the amino terminus is the region that was determined to be disordered in the references cited above. (c) Histone H3 (Swiss-Prot: P68431). The structure of chicken H3 in a histone octamer complex (PDB: 2HIO) was determined by X-ray methods for residues 1–135. Residues 1–42 are missing, presumably due to disorder . (d) Sm D1 (Swiss-Prot: P62314). The structure of a protein complex between Sm D1 (residues 2–119) and Sm D2 was studied by X-ray methods (PDB: 1B34) . Residues 82–119 from Sm D1 are missing from the structure.