|
Gene
|
Forward primer
|
Sequence
|
Reverse primer
|
Sequence
|
Annealing temperature (°C)
|
|---|
|
Sequencing primers
|
|
FCGR2A
|
IIA-SF
|
dGGAGAAACCATCATGCTGAG
|
IIA-SR
|
dCAATTTTGCTGCTATGGGC
|
52
|
|
FCGR2B (E3)
|
IIB-E3SF
|
dGGCATCTCAAGCTCC
|
IIB-E3SR
|
dAGAGTCACAGAGTCCTCC
|
58
|
|
FCGR2B (E6)
|
IIB-E6SF
|
dCCCATCCAACCCTGG
|
IIB-E6SR
|
dGGCAGATTCCTCAGCAAATCA
|
56
|
|
FCGR2B (3'UTR)
|
IIB-UTRSF
|
dTGGGGAGGACAGGGAGAT
|
IIB-UTRSR
|
dATCACTTTTAATGTGCTGGTAGAGG
|
63
|
|
Genotyping primers
|
|
FCGR2A
|
PCR1
|
dGGAGAAACCATCATGCTGAG
|
4INM
|
dCAATTTTGCTGCTATGGGC
|
52
|
| |
IIA-R
|
dAATCCCAGAAATTCTCCCG
|
IIA-H
|
dAATCCCAGAAATTCTCCCA
| |
|
FCGR3B-NA1
|
IIIB-NA1F
|
dCAGTGGTTTCACAATGTGAA
|
IIIB-NA1R
|
dATGGACTTCTAGCTGCACCG
|
54
|
| |
IIIB-NA1PR
|
dGTCTCTTTCTGCTTGGTGATGG
|
IIIB-NA1PR
|
dTTTTCCCCTCTAAACTGGG
| |
|
FCGR3B-NA2
|
IIIB-NA2F
|
dCTCAATGGTACAGCGTGCTT
|
IIIB-NA2R
|
dCTGTACTCTCCACTGTCGTT
|
62
|
| |
IIIB-NA2PR
|
dCTGGCTTGCTGATGAAGATAC
|
IIIB-NA2PR
|
dGTAACGCTTNGGCACCACC
| |
|
FCGR2B
|
IIB-UTRSF
|
dTGGGGAGGACAGGGAGAT
|
IIB-UTRGR
|
dCAGAAGGTGCAGTCGGC
|
50
|
- The mutation inserted into the FCGR2B reverse nested primer (IIB-UTRGR) is shown in italics. This introduced an allele-specific HaeIII restriction site (GGCC) in the presence of the G but not the A allele.
