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Table 1 Primer pairs used to amplify specific FCGR sequencing templates

From: Analysis of Fcγ receptor haplotypes in rheumatoid arthritis: FCGR3A remains a major susceptibility gene at this locus, with an additional contribution from FCGR3B

Gene Forward primer Sequence Reverse primer Sequence Annealing temperature (°C)
Sequencing primers
   FCGR2A IIA-SF dGGAGAAACCATCATGCTGAG IIA-SR dCAATTTTGCTGCTATGGGC 52
   FCGR2B (E3) IIB-E3SF dGGCATCTCAAGCTCC IIB-E3SR dAGAGTCACAGAGTCCTCC 58
   FCGR2B (E6) IIB-E6SF dCCCATCCAACCCTGG IIB-E6SR dGGCAGATTCCTCAGCAAATCA 56
   FCGR2B (3'UTR) IIB-UTRSF dTGGGGAGGACAGGGAGAT IIB-UTRSR dATCACTTTTAATGTGCTGGTAGAGG 63
Genotyping primers
   FCGR2A PCR1 dGGAGAAACCATCATGCTGAG 4INM dCAATTTTGCTGCTATGGGC 52
  IIA-R dAATCCCAGAAATTCTCCCG IIA-H dAATCCCAGAAATTCTCCCA  
   FCGR3B-NA1 IIIB-NA1F dCAGTGGTTTCACAATGTGAA IIIB-NA1R dATGGACTTCTAGCTGCACCG 54
  IIIB-NA1PR dGTCTCTTTCTGCTTGGTGATGG IIIB-NA1PR dTTTTCCCCTCTAAACTGGG  
   FCGR3B-NA2 IIIB-NA2F dCTCAATGGTACAGCGTGCTT IIIB-NA2R dCTGTACTCTCCACTGTCGTT 62
  IIIB-NA2PR dCTGGCTTGCTGATGAAGATAC IIIB-NA2PR dGTAACGCTTNGGCACCACC  
   FCGR2B IIB-UTRSF dTGGGGAGGACAGGGAGAT IIB-UTRGR dCAGAAGGTGCAGTCGGC 50
  1. The mutation inserted into the FCGR2B reverse nested primer (IIB-UTRGR) is shown in italics. This introduced an allele-specific HaeIII restriction site (GGCC) in the presence of the G but not the A allele.