Table 1 Primer pairs used to amplify specific FCGR sequencing templates
Gene | Forward primer | Sequence | Reverse primer | Sequence | Annealing temperature (°C) |
|---|---|---|---|---|---|
Sequencing primers | |||||
FCGR2A | IIA-SF | dGGAGAAACCATCATGCTGAG | IIA-SR | dCAATTTTGCTGCTATGGGC | 52 |
FCGR2B (E3) | IIB-E3SF | dGGCATCTCAAGCTCC | IIB-E3SR | dAGAGTCACAGAGTCCTCC | 58 |
FCGR2B (E6) | IIB-E6SF | dCCCATCCAACCCTGG | IIB-E6SR | dGGCAGATTCCTCAGCAAATCA | 56 |
FCGR2B (3'UTR) | IIB-UTRSF | dTGGGGAGGACAGGGAGAT | IIB-UTRSR | dATCACTTTTAATGTGCTGGTAGAGG | 63 |
Genotyping primers | |||||
FCGR2A | PCR1 | dGGAGAAACCATCATGCTGAG | 4INM | dCAATTTTGCTGCTATGGGC | 52 |
IIA-R | dAATCCCAGAAATTCTCCCG | IIA-H | dAATCCCAGAAATTCTCCCA | ||
FCGR3B-NA1 | IIIB-NA1F | dCAGTGGTTTCACAATGTGAA | IIIB-NA1R | dATGGACTTCTAGCTGCACCG | 54 |
IIIB-NA1PR | dGTCTCTTTCTGCTTGGTGATGG | IIIB-NA1PR | dTTTTCCCCTCTAAACTGGG | ||
FCGR3B-NA2 | IIIB-NA2F | dCTCAATGGTACAGCGTGCTT | IIIB-NA2R | dCTGTACTCTCCACTGTCGTT | 62 |
IIIB-NA2PR | dCTGGCTTGCTGATGAAGATAC | IIIB-NA2PR | dGTAACGCTTNGGCACCACC | ||
FCGR2B | IIB-UTRSF | dTGGGGAGGACAGGGAGAT | IIB-UTRGR | dCAGAAGGTGCAGTCGGC | 50 |