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Table 3 Reactivity in ELISA of sera from mothers of groups I and II

From: 52-kDa Ro/SSA epitopes preferentially recognized by antibodies from mothers of children with neonatal lupus and congenital heart block

Antigen Systemic lupus erythematosus Sjögren's syndrome Asymptomatic
  Group I (%) Group II (%) P Group I (%) Group II (%) P Group I (%)
Recombinant Ro52 protein 97 66 0.009* 67 98 0.027* 31
1–13 MASAARLTMMWEE 77 46 0.084 47 58   31
10–25 MWEEVTPICLDPFVE 0 14   0 0   0
12–35 EEVTCPICLDPFVEPVSIECGHSF 10 16   20 8   13
25–40 EPVSIECGHSFCQECI 0 13   10 0   6
34–56 SFCQECISQVGKGGGSVCAVCRQ 0 1   7 0   13
38–53 ECISQVGKGGGSVCAV 3 4   7 0   0
50–64 VCAVCRQRFLLKNLR 25 3   13 5   6
63–79 LRPNRQLANMVNNLKEI 0 6   0 3   0
77–94 KEISQEAREGTQGERCAV 18 1   3 0   0
93–116 AVHGERLHLFCEKDGKALCWVCAQ 40 12   10 9   0
94–109 VHGERLHLFCEKDGKA 3 13   0 0   0
107–122 GKALCWVCAQSRKHRD 69 28 0.023* 47 33   13
107–126 GKALCWVCAQSRKHRDHAMV 37 7 0.062 13 8   13
121–137 RDHAMVPLEEAAQEYQE 0 4   0 0   0
135–151 YQEKLQVALGELRRKQE 3 14   0 0   0
149–166 KQELAEKLEVEIAIKRAD 0 12   0 1   0
164–180 RADWKKTVETQKSRIHA 0 9   0 0   6
178–193 IHAEFVQQKNFLVEEE 3 14   0 0   0
191–208 EEEQRQLQELEKDEREQL 0 0   0 0   0
206–224 EQLRILGEKEAKLAQQSQA 0 1   7 0   9
222–235 SQALQELISELDRRCHS 17 3   23 1   6
236–250 CHSSALELLQEVIIV 27 6   10 7   0
249–264 IVLERSESWNLKDLDI 0 5   7 0   0
262–279 LDITSPELRSVCHVPGLK 0 0   0 0   0
277–292 GLKKMLRTCAVHITLD 83 49 0.049* 40 24   6
290–304 TLDPDTANPWLILSE 0 7   7 0   0
302–317 LSEDRRQVRLGDTQQS 10 2   7 0   0
314–328 TQQSIPGNEERFDSY 0 9   0 9   0
326–340 DSYPMVLGAQHFHSG 0 0   0 0   0
337–352 FHSGKHYWEVDVTGKE 23 11   3 12   6
350–367 GKEAWDLGVCRDSVRRKG 0 1   0 0   9
365–382 RKGHFLLSSKSGFWTIWL 77 47 0.054 50 33   25
380–396 IWLWNKQKYEAGTYPQT 0 5   0 2   0
394–412 PQTPLHLQVPPCQVGIFLD 18 1   3 10   0
411–423 LDYEAGMVSFYNI 0 2   0 0   0
422–438 NITDHGSLIYSFSECAF 16 2   0 0   0
436–451 CAFTGPLRPFFSPGFN 13 7   13 10   0
447–463 SPGFNDGGKNTAPLTLC 8 9   0 6   0
463–475 CPLNIGSQGSTDY 0 7   7 1   0
Ro60 PROTEIN 80 67   67 99 0.022* 31
1–23 MEESVNQMQPLNEKQIANSQDGY 15 6   17 1   6
18–38 NSQDGYVWQVTDMNRLHRFLC 32 11   20 1 0.097 0
21–41 DGYVWQVTDMNRLHRFLCFGS 64 27 0.044* 23 38   0
304–324 VCEKLCNEKLLKKARIHPFHI 50 18   27 4   13
495–518 KLIVCGMTSNGFTIADPDDRGMLD 10 24 0.058 7 11 0.079 13
524–538 TGALDVIRNFTLDMI 10 17   3 9   0
La PROTEIN 93 30 <0.0001* 40 68   13
  1. The 192 samples described in Table 1 were systematically tested in at least two independent tests. Only the IgG response was tested. Patients' sera were diluted 1:1,000. To be tested in the ELISA, peptide 21–41 of Ro60 protein was conjugated to ovalbumin. The sera were considered positive when optical density ≥0.3. Statistically significant differences between groups were determined by Student's t test. *P < 0.05 considered significant.
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Arthritis Research & Therapy

ISSN: 1478-6362

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