IL-8, MCP-1 and IP-10 production by dermal fibroblasts following contact with T-helper cells. Normal fibroblasts were plated at 2 × 104 cells/well; 72 hours later the culture medium was replaced and T cell membranes generated from resting or activated Th1 and Th2 clones were added to the wells to a final volume of 200 μl. Chemokine levels were determined in the supernatants by enzyme-linked immunosorbent assay after 48 hours of further culture. (a) The points represent the mean ± standard deviation of triplicate cultures. Similar results were obtained in an additional experiment. (b) T cell membranes corresponding to 2 × 105 cells generated from activated Th1 (n = 10) and Th2 (n = 10) clones were added to the wells in triplicate cultures. The bars represent the mean ± standard error. IL, interleukin; IP, interferon-γ inducible protein; MCP, monocyte chemoattractant protein; Th, T-helper.