Figure 7From: Enumeration and phenotypical analysis of distinct dendritic cell subsets in psoriatic arthritis and rheumatoid arthritisCytokine release and phenotypic changes following mDC exposure to TLR agonist. Synovial fluid (SF) myeloid dendritic cells (mDCs) mature and release tumour necrosis factor alpha (TNF-α) at comparable levels with healthy peripheral blood (PB) mDCs, but only SF mDCs release IL-10. Cytokine release and the maturation status of mDCs purified from healthy control PB (n = 3) or inflammatory arthritis SF (rheumatoid arthritis, n = 2; psoriatic arthritis, n = 1) were assessed after 24-hour incubation with granulocyte–macrophage colony-stimulating factor (GM-CSF)-supplemented media alone or containing Staphylococcus aureus peptidoglycan (PGN) (10 μg/ml). (a) The concentrations of TNF-α and IL-10 in supernatant from the cultures were determined by multiplex analysis and were expressed as release per 5 × 104 cells. Squares indicate individual donors. (b) Maturation and co-stimulation marker expression was determined by flow cytometry, and one representative example from each of the healthy control PB and inflammatory arthritis SF groups are shown.Back to article page