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Table 1 Peptide sequence confirmation of bovine fibroblast activation protein alpha from chondrocyte membranes.

From: Fibroblast activation protein alpha is expressed by chondrocytes following a pro-inflammatory stimulus and is elevated in osteoarthritis

m/z (Charge) Locationa Sequence
564.78 (2+) 335–343 TQEHIEESR
736.77 (2+) 367–375 IFSDKDGYK
611.30 (3+) 210–219 YALWWSPNGK
751.41 (2+) 162–173 LAYVYQNNIYLK
796.41 (2+) 592–605 LGVYEVEDQITAVR
969.56 (2+) 534–550 YPLLIQVYGGPCSQSVR
697.41 (2+) 510–521 LKVDDITLWYK
786.47 (2+) 551–564 SIFAVSWISYLASK
715.65 (3+) 144–161 NELPRPIQYLCWSPVGSK
776.36 (3+) 124–142 YSYTATYHIYDLTNGEFIR
1098.96 (2+) 403–421 VTQDSLFYSSNEFEGYPGR
  1. The 97 kDa band obtained following biotinylated fluorophosphonate labelling of bovine chondrocytes was trypsin digested and analysed using mass spectrometry; 11 separate peptides were detected. aNumbering according to uniprot entry Sepr_Human. Human has Y not H in position 131, G143 is deleted in the bovine sequence, human has E not G in position 417, E not K at 511, E not D at 514, V not I at 552, and N not S at 556.