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Table 1 Peptide sequence confirmation of bovine fibroblast activation protein alpha from chondrocyte membranes.

From: Fibroblast activation protein alpha is expressed by chondrocytes following a pro-inflammatory stimulus and is elevated in osteoarthritis

m/z (Charge)

Locationa

Sequence

564.78 (2+)

335ā€“343

TQEHIEESR

736.77 (2+)

367ā€“375

IFSDKDGYK

611.30 (3+)

210ā€“219

YALWWSPNGK

751.41 (2+)

162ā€“173

LAYVYQNNIYLK

796.41 (2+)

592ā€“605

LGVYEVEDQITAVR

969.56 (2+)

534ā€“550

YPLLIQVYGGPCSQSVR

697.41 (2+)

510ā€“521

LKVDDITLWYK

786.47 (2+)

551ā€“564

SIFAVSWISYLASK

715.65 (3+)

144ā€“161

NELPRPIQYLCWSPVGSK

776.36 (3+)

124ā€“142

YSYTATYHIYDLTNGEFIR

1098.96 (2+)

403ā€“421

VTQDSLFYSSNEFEGYPGR

  1. The 97 kDa band obtained following biotinylated fluorophosphonate labelling of bovine chondrocytes was trypsin digested and analysed using mass spectrometry; 11 separate peptides were detected. aNumbering according to uniprot entry Sepr_Human. Human has Y not H in position 131, G143 is deleted in the bovine sequence, human has E not G in position 417, E not K at 511, E not D at 514, V not I at 552, and N not S at 556.