Changes in splenic mononuclear cells as a result of treatment with the ethyl acetate (EA) extract of Tripterygium wilfordii Hook F (TwHF). (a) Flow cytometric analysis of spleen mononuclear cells from NZB/WF1 mice after treatment with vehicle or the EA extract of TwHF. Horizontal bars indicate the median value for each group. Numbers of mice analyzed are indicated in parentheses. *p < 0.05, EAhigh versus vehicle. (b) Immunohistochemical analysis of spleen from NZB/WF1 mice after treatment with vehicle (A,C) or the EA extract of TwHF (B,D). CD3+ cells (red) and CD11c+ cells (green) are shown in (A,B,C) and IgD+ cells shown in (D,E,F). Results shown are representative of five mice per group. Original magnification × 400.