Immunoblot analysis of Galectin-3 in supernatant from cultured synoviocytes or plasma from arthritic rats. Western blot analysis was performed using an anti-Galectin-3 antibody on (a) supernatant from cultured tissue synoviocytes or (b) plasma samples collected at the indicated time points from arthritic rats. Two nanograms of recombinant mouse Galectin-3 (R&D; cat. no. 1197-GA) was loaded as the positive control (lane c). Arrow denotes rat Galectin-3; asterisk denotes non-specific protein band.