Inhibitory effects of αvβ3-retargeted adenovirus on tumor necrosis factor (TNF)-α induced gene expression of cell adhesion molecules, cytokines, and angiogenesis associated molecules in human umbilical vein endothelial cells (HUVECs). Non-transduced (solid bar) and AddnIκB-PEG-RGD transduced HUVECs (open bar) with 7,500 vp/cell were activated with TNF-α (100 ng/ml) for 24 h. Real time RT-PCR was performed on mRNA isolated from each respective HUVEC incubation. Data were normalized to untreated, non-activated control HUVECs arbitrarily set at 1. Results are expressed as the mean ± standard deviation (n = 3). Asterisks indicate p < 0.05 compared with respective control cells without activation with TNF-α (TNF-α (-)). ICAM, intercellular adhesion molecule; nd, not detectable; ns, not significant; VCAM, vascular cell adhesion molecule; VEGF, vascular endothelial growth factor.