Figure 5

AddnIκB-PEG-RGD can transduce functional dnIκB in a concentration dependent way leading to diminished intercellular adhesion molecule (ICAM)-1 protein expression upon tumor necrosis factor (TNF)-α stimulation in human umbilical vein endothelial cells (HUVECs) as determined by flow cytometry. Minus and plus signs and denotes resting, non-infected, and TNF-α activated, non-infected HUVECs, respectively. All other histograms represent the responses of dnIκB expressing HUVECs to TNF-α activation. The larger the number of viral particles/cell (× 10³) of AddnIκB-PEG-RGD used for the infection, the higher the percentage of ICAM-1dim cells upon TNF-α activation.