Figure 4

Ets-1 and Fli-1 bind the CCN2 promoter. A double-stranded oligomer corresponding to the -126 to -77 segment of the CCN2 promoter was used in a gel shift assay with NIH 3T3 fibroblast nuclear extract (5 μg) in the presence or absence of 100-fold molar excess of specific competitor corresponding to unlabeled probe (probe), or competitors corresponding to a consensus ETS (ETS) or NFκB binding site, or a 1 h pre-incubation with specific anti-Ets-1 antibody (Ets-1), anti-fli-1 (Fli-1), anti-Elk-1 (Elk-1) or anti-Sp1 (Sp1) antibody. Location of the free probe and shifted Ets-1 containing complex (arrow) are indicated. Representative gel shift assays are shown (N = 3).