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Figure 2 | Arthritis Research & Therapy

Figure 2

From: CD147 overexpression on synoviocytes in rheumatoid arthritis enhances matrix metalloproteinase production and invasiveness of synoviocytes

Figure 2

Gelatin zymography of culture medium conditioned by fibroblast-like synoviocytes (FLS) and THP-1 cells. Cells were cultured in serum-free media for 24 hours, and the conditioned media were collected and analyzed for matrix metalloproteinase (MMP) activity by gelatin zymography. (a) Secretion and activation of MMPs in FLS from rheumatoid arthritis (RA) and osteoarthritis (OA) patients, THP-1 cells, RA FLS co-cultured with THP-1 cells and in co-cultured cells treated by AP-9 or SSP, and ethylenediamine tetraacetic acid or phenanthroline. Top two bands, MMP-9 gelatinase; lower two bands, MMP-2 gelatinase. (b) The value of proMMP-2 and MMP-2 activity of RA FLS was higher than that of OA FLS (six samples with RA FLS, four samples with OA FLS; P < 0.05). (c) The value of proMMP-9, MMP-9 and proMMP-2 activity of differentiated THP-1 cells (dTHP-1) was higher than that of undifferentiated THP-1 cells (uTHP-1) (n = 6, P < 0.05). (d) The value of proMMP-9, MMP-9 and proMMP-2 activity of RA FLS co-cultured with uTHP-1 was higher than that of co-cultured cells treated by AP-9 (n = 6, P < 0.05), while no inhibitory effects were found in irrelevant peptide SSP (n = 6, P > 0.05). (e) The value of proMMP-9, MMP-9, proMMP-2 and MMP-2 activity of RA FLS co-cultured with dTHP-1 was higher than that of co-cultured cells treated by AP-9 (n = 6, P < 0.05), while no inhibitory effects were found in irrelevant peptide SSP (n = 6, P > 0.05). Values are the means ± standard error (n = 4–6). *P < 0.05 (experimental groups versus control groups).

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