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Table 1 Results of expressions of CD147 on fibroblast-like synoviocytes (FLS) and monocytes/macrophages

From: CD147 overexpression on synoviocytes in rheumatoid arthritis enhances matrix metalloproteinase production and invasiveness of synoviocytes

  Osteoarthritis FLS RA FLS Undifferentiated THP-1 cells PMA-induced differentiated THP-1 cells Monocytes/macrophages from peripheral blood of healthy humans Monocytes/macrophages from synovial fluid of RA patients
Percentage of positive staining cells (%) 88.75 ± 2.32 97.17 ± 0.6* 99.23 ± 0.47 99.33 ± 0.38 99.18 ± 0.55 99.27 ± 0.39
Mean fluorescence intensity 192.72 ± 12.67 193.33 ± 15.83 202.1 ± 19.25 286.61 ± 31.63# 78.32 ± 18.25 136.37 ± 28.16
Number 4 6 6 6 10 10
  1. Values presented as the mean ± standard error (n = 4–10 independent samples per group). Expressions of CD147 on FLS from osteoarthritis or rheumatoid arthritis (RA) patients and monocytes/macrophages (including undifferentiated THP-1/phorbol 12-myristate 13-acetate [PMA]-induced differentiated dTHP-1 cells, monocytes/macrophages from synovial fluid of rheumatoid arthritis patients or from peripheral blood of healthy humans) were measured by flow cytometry using fluorescein isothiocyanate-labeled anti-CD147 mAb. Analysis was conducted on a FACS™ brand flow cytometer. Two parameters of FLS and THP-1 cells were used: the percentage of positive staining cells, and the mean fluorescence intensity (denoting the quantity of CD147 expression on the surface of each cell).
  2. *P < 0.05 versus OA FLS; #P < 0.05 versus undifferentiated THP-1 cells; P < 0.05 versus monocytes from peripheral blood of healthy humans.