CD147 overexpression on synoviocytes in rheumatoid arthritis enhances matrix metalloproteinase production and invasiveness of synoviocytes

Table 1 Results of expressions of CD147 on fibroblast-like synoviocytes (FLS) and monocytes/macrophages

	Osteoarthritis FLS	RA FLS	Undifferentiated THP-1 cells	PMA-induced differentiated THP-1 cells	Monocytes/macrophages from peripheral blood of healthy humans	Monocytes/macrophages from synovial fluid of RA patients
Percentage of positive staining cells (%)	88.75 ± 2.32	97.17 ± 0.6*	99.23 ± 0.47	99.33 ± 0.38	99.18 ± 0.55	99.27 ± 0.39
Mean fluorescence intensity	192.72 ± 12.67	193.33 ± 15.83	202.1 ± 19.25	286.61 ± 31.63^#	78.32 ± 18.25	136.37 ± 28.16^‡
Number	4	6	6	6	10	10

Values presented as the mean ± standard error (n = 4–10 independent samples per group). Expressions of CD147 on FLS from osteoarthritis or rheumatoid arthritis (RA) patients and monocytes/macrophages (including undifferentiated THP-1/phorbol 12-myristate 13-acetate [PMA]-induced differentiated dTHP-1 cells, monocytes/macrophages from synovial fluid of rheumatoid arthritis patients or from peripheral blood of healthy humans) were measured by flow cytometry using fluorescein isothiocyanate-labeled anti-CD147 mAb. Analysis was conducted on a FACS™ brand flow cytometer. Two parameters of FLS and THP-1 cells were used: the percentage of positive staining cells, and the mean fluorescence intensity (denoting the quantity of CD147 expression on the surface of each cell).
*P < 0.05 versus OA FLS; ^#P < 0.05 versus undifferentiated THP-1 cells; ^‡P < 0.05 versus monocytes from peripheral blood of healthy humans.

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