DCs accumulate with age in mice with lupus. (a) To study DCs, we analyzed the total population of splenocytes in mice 6 to 8 weeks old (young) and in mice 6 to 9 months old (old) and gated for CD11c+ cells that were negative for the B cell marker CD19. (b,c) Percentages (b) and absolute numbers (c) of DCs gated as in (a). Each circle represents the percentage or absolute number of CD11c+ cells in one individual mouse, and the horizontal bars represent the average of each group. Results of the Mann-Whitney test analyses are shown. N.S. indicates p > 0.05.