| |
Wild-type mice (nM ± SEM)
|
A2A knockout mice (nM ± SEM)
|
A3 knockout mice (nM ± SEM)
|
|---|
|
Control
|
118 ± 6 (n = 19)
|
110 ± 6 (n = 14)
|
133 ± 6 (n = 12)
|
|
Methotrexate (0.75 mg/kg/week)
|
178 ± 12* (n = 15)
|
162 ± 7** (n = 7)
|
214 ± 10† (n = 9)
|
- Wild-type mice, A2A receptor knockout mice or A3 receptor knockout mice were treated with either weekly injections of methotrexate (0.75 mg/kg) or saline control for 4 weeks prior to induction of inflammation. Inflammatory exudates were induced in the peritoneum of mice, as described. After 4 hours the exudates were collected and the adenosine levels quantitated. Wild-type data are a combination from both mouse strains. *P < 0.0001 vs wild-type control mice, Student's t test; **P < 0.0001 vs A2A knockout control mice, Student's t test; † P < 0.0001 vs A3 knockout control mice, Student's t test.
