Suppression of inflammation by low-dose methotrexate is mediated by adenosine A2A receptor but not A3receptor activation in thioglycollate-induced peritonitis

Table 4 IL-10 concentration in peritoneal exudates

	Wild-type mice (pg/ml ± SEM)	A_2A knockout mice (pg/ml ± SEM)	A₃ knockout mice (pg/ml ± SEM)
Control	62 ± 7 (n = 24)	73 ± 9 (n = 12)	115 ± 14** (n = 15)
Methotrexate (0.75 mg/kg/week)	97 ± 18* (n = 12)	41 ± 6^† (n = 7)	150 ± 31 (n = 7)

Wild-type mice, A_2A receptor knockout mice or A₃ receptor knockout mice were treated with either weekly injections of methotrexate (0.75 mg/kg) or saline control for 4 weeks prior to induction of inflammation. Inflammatory exudates were induced in the peritoneum of mice, as described. After 4 hours the exudates were collected, centrifuged at 100 × g and frozen. IL-10 levels were later quantitated by ELISA. Wild-type data are a combination from both mouse strains. ** P < 0.001 vs wild-type control mice, Student's t test; * P < 0.05 vs wild-type control mice, Student's t test; ^†P < 0.05 vs A_2A knockout control mice, Student's t test.

ISSN: 1478-6362