Skip to main content

Table 4 IL-10 concentration in peritoneal exudates

From: Suppression of inflammation by low-dose methotrexate is mediated by adenosine A2A receptor but not A3receptor activation in thioglycollate-induced peritonitis

  Wild-type mice (pg/ml ± SEM) A2A knockout mice (pg/ml ± SEM) A3 knockout mice (pg/ml ± SEM)
Control 62 ± 7 (n = 24) 73 ± 9 (n = 12) 115 ± 14** (n = 15)
Methotrexate (0.75 mg/kg/week) 97 ± 18* (n = 12) 41 ± 6 (n = 7) 150 ± 31 (n = 7)
  1. Wild-type mice, A2A receptor knockout mice or A3 receptor knockout mice were treated with either weekly injections of methotrexate (0.75 mg/kg) or saline control for 4 weeks prior to induction of inflammation. Inflammatory exudates were induced in the peritoneum of mice, as described. After 4 hours the exudates were collected, centrifuged at 100 × g and frozen. IL-10 levels were later quantitated by ELISA. Wild-type data are a combination from both mouse strains. ** P < 0.001 vs wild-type control mice, Student's t test; * P < 0.05 vs wild-type control mice, Student's t test; P < 0.05 vs A2A knockout control mice, Student's t test.