Figure 14

Time-kinetic effect of silencing nuclear factor (NF)κB1 mRNA in bone marrow CD34+ cells from patients with rheumatoid arthritis on the viable cell counts and the production of β₂-microblobulin (β₂MG). Purified bone marrow CD34+ cells from patients with rheumatoid arthritis were transfected with small interfering RNA (siRNA) for NFκB1 or scrambled sequence control siRNA, after which the cells were further incubated in culture medium with stem cell factor (10 ng/ml), granulocyte-macrophage colony stimulating factor (1 ng/ml) and tumor necrosis factor-α (10 ng/ml) up to 4 weeks with no medium changes. After various periods of incubation (W, weeks), the cells were counted and the quantities of β₂MG in the culture supernatants were determined by ELISA. This is the same experment as shown in Figure 13. Data are representative of three different experiments.