Figure 2

Expression of nuclear factor (NF)κB1 (p50) protein in bone marrow CD34+ cells. Purified bone marrow CD34+ cells from a rheumatoid arthritis patient were permeabilized and then stained with phychoerythrin-conjugated anti-NFκB p50 monoclonal antibody or phychoerythrin-conjugated normal mouse IgG1, followed by analysis with flow cytometry. The level of NFκB1 protein was expressed by mean fluorescence intensity as described in Materials and methods.