Figure 7
Effect of tumor necrosis factor (TNF)-α on the expression of mRNAs for nuclear factor (NF)κB1 (p50), NFκB2 (p52) and RelA (p65) in bone marrow CD34+ cells. Bone marrow CD34+ cells from healthy individuals were incubated in culture medium with or without TNF-α (10 ng/ml) for 24 hours. After the incubation, total RNA was isolated for evaluation of the expression of mRNAs for NFκB1, NFκB2, RelA and β-actin by real-time quantitative PCR. The data are expressed as the ratio of the mRNA copy numbers to those of β-actin. The data are representative of two different experiments.