Figure 8

Silencing of nuclear factor (NF)κB1 mRNA in bone marrow CD34+ cells by small interfering RNA (siRNA) for NFκB1. Purified bone marrow CD34+ cells were transfected with siRNA for NFκB1 or a scrambled sequence control siRNA after a 24 hours incubation in culture medium with stem cell factor (10 ng/ml) and granulocyte-macrophage colony stimulating factor (1 ng/ml). After the transfection, the cells were further incubated for 48 hours in culture medium with stem cell factor and granulocyte-macrophage colony stimulating factor, and total RNA was isolated for evaluation of the expression of NFκB1 mRNA and β-actin mRNA by real-time quantitative PCR. The data are expressed as the ratio of the mRNA copy numbers to those of β-actin.