Figure 9

Inhibition of the generation of fibroblast-like cells by silencing nuclear factor (NF)κB1 mRNA in bone marrow CD34+ cells from patients with rheumatoid arthritis. Purified bone marrow CD34+ cells were transfected with small interfering RNA (siRNA) for NFκB1 or a scrambled sequence control, after which the cells were incubated in culture medium with stem cell factor (10 ng/ml), granulocyte-macrophage colony stimulating factor (1 ng/ml) and tumor necrosis factor-α (10 ng/ml) for 4 weeks with no medium changes. Morphological changes were observed under light microscopy (original magnification, ×20; inset, ×50 magnification). The data are representative of 12 different experiments.