Table 1 Primer sequences used in real-time quantitative PCR for analysis of mRNA for various nuclear factor κB components
Gene product (GenBank accession no.) | Primer sequences | Nucleotides |
|---|---|---|
NFκB1 [M58603] | Forward: 5'-TCC ACA AGG CAG CAA ATA GA-3' | 3,125–3,144 |
Reverse: 5'-GGG GCA TTT TGT TGA GAG TT-3' | 3,244–3,263 | |
NFκB2 [NM_002502] | Forward: 5'-TTC TGA AGG CTG GTG CTG AC-3' | 2,220–2,239 |
Reverse: 5'-AGT GAG GTC AAG AGG CGT GT-3' | 2,352–2,371 | |
RelA [NM_021925] | Forward: 5'-GAA GAA GAG TCC TTT CAG CG-3' | 1,011–1,030 |
Reverse: 5'-GGG AGG ACG TAA AGG GAT AG-3' | 1,116–1,135 | |
β-actin [X00351] | Forward: 5'-GCA AAG ACC TGT ACG CCA AC-3' | 910–929 |
Reverse: 5'-CTA GAA GCA TTT GCG GTG GA-3' | 1,150–1,169 |