Immune complexes from rheumatoid arthritis synovial fluid induce FcγRIIa dependent and rheumatoid factor correlated production of tumour necrosis factor-α by peripheral blood mononuclear cells

Table 1 Correlations between rheumatoid factor or anti-CCP and IgG content in immune complexes and TNF-α inducing properties of immune complexes

Correlation	Study 1 (n = 15)		Study 2 (n = 32)
	r	P	r	P
RF/SF immune complexes
IgG in SF precipitates/TNF-α induced by SF precipitates	0.604	0.0239	0.503	0.0051
RF/TNF-α induced by SF precipitates	0.404	0.1310 (NS)	0.594	0.001
RF/IgG in SF precipitates	0.729	0.0064	0.360	0.0449
RF/serum immune complexes
IgG in serum precipitates/TNF-α induced by serum precipitates	0.171	NS	-0.265	NS
RF/TNF-α induced by serum precipitates	0.332	NS	0.329	NS
RF/IgG in serum precipitates	0.346	NS	0.388	0.0308
Anti-CCP/SF immune complexes
Anti-CCP/TNF-α induced by SF precipitates	ND	ND	0.043	NS
Anti-CCP/IgG in SF precipitates	ND	ND	0.083	NS
Anti-CCP/serum immune complexes
Anti-CCP/TNF-α induced by serum precipitates	ND	ND	0.191	NS
Anti-CCP/IgG in serum precipitates	ND	ND	0.246	NS

CCP, cyclic citrullinated peptide; ND, not done; NS, not significant; RA, rheumatoid arthritis; RF, rheumatoid factor; SF, synovial fluid; TNF, tumour necrosis factor.