Figure 2

Sox gene expression in monolayer and cell aggregate cultures. Gene expression for monolayer cells (black bars) between P2 and P3 in standard medium (n = 3), for cell aggregate (white open bars) culture in chondrogenic differentiation medium after 14 days of culture under normoxia (n = 3). Gene expression for monolayer cells (light grey bars) between P2 and P3 in standard medium (n = 3) and for cell aggregate (dark grey bars) in chondrogenic differentiation medium after 14 days of culture under low oxygen tension (n = 3). *p < 0.05; **p < 0.001 (Student's unpaired t test) in cell aggregates from fibroblast growth factor 2 (FGF2)-expanded versus non-FGF2-expanded cells. P2, passage 2; P3, passage 3.