Table 2 Primers and probes for used allelic discrimination chemistry
Forward and reverse primers (5' to 3') | Fluorescent-labeled probes (5' to 3') |
|---|---|
SNP1F GCCCAGCTAATTTTGTTTTGTTTTGTT | SNP1 allele1 VIC- TTGCAGCGTTGCCCA-MGB |
SNP1R GGAGTATCGTTTGAGGCTAAGAGTT | SNP1 allele2 FAM- TATTGCAGTGTTGCCCA-MGB |
SNP2F TCCTAGCTCTGGGCATACGAAT | SNP2 allele1 VIC-CCACTTATCTATAGAGCTT-MGB |
SNP2R AGCTGTATGCCTATGGAGCCTTA | SNP2 allele2 FAM-CACTTATCTGTAGAGCTT-MGB |
SNP4F CTGGCAATTCTGCCTTGTTTCAG | SNP4 allele1VIC- CCGAAGATAAAAGAATC-MGB |
SNP4R GGATTACAGCCGTGAGCCA | SNP4 allele2 FAM- CGAAGATAGAAGAATC-MGB |
SNP5F AAGCTGAGGCAGGAAGATCAC | SNP5 allele1 VIC- TCGGGAGTTCGAGACC-MGB |
SNP5R ACACAGGGTTTCACCATGCT | SNP5 allele2 FAM- CGGGAGTTGGAGACC-MGB |
SNP6F TCCCTACTGTTGTTTCCGCC | SNP6 allele1VIC-TGAAGACCCTGGGC-MGB |
SNP6R CCCGAAGTCCGAGCACC | SNP6 allele2 FAM-TGAAGACCCCGGGC-MGB |
SNP9F GAAAGTTTTAACACTGGAAACTGCAA | SNP9 allele1 VIC-TTTTGGTAGCCCTCTC-MGB |
SNP9R TTACACTTTCTGCAACAGAAAGTAAGC | SNP9 allele2 FAM-TTTTGGTATCCCTCTCC-MGB |
SNP11F ACAGGTTTTGGAAGGCACAGA | SNP11 VIC- ACGGAAGAAAAGATTT-MGB |
SNP11R AATAAAGTGGCAGAGGATACGAGTACT | SNP11 FAM-ACGGAAGAAAACATTT-MGB |
SNP12F AATTGTCTCCCAGTGCATTTTGC | SNP12 allele1 VIC-CTGCCAACTCTGGCTG-MGB |
SNP12R TCTTCCCGAAGCTGTGTAGACT | SNP12 allele2 FAM- CCAACGCTGGCTG-MGB |
SNP13F AATTGTCTCCCAGTGCATTTTGC | SNP13 allele1 VIC-CAGCCGCTTTAGC-MGB |
SNP13R TCTTCCCGAAGCTGTGTAGACT | SNP13 allele1 FAM- CAGCCACTTTAGC-MGB |
SNP14F AGTTTAAACCATGTCTCAAGATCTCTGC | SNP14 allele1 VIC-TTGTGCTACAATTATT-MGB |
SNP14R AGTACTTGTGACTCTGTCATTAATAGAAATACCT | SNP14 allele2 VIC-TTGTGCTACAGTTATT-MGB |