Table 1 Summary of primers used
cDNA | Forward and reverse primers | Ta (°C) | Product (bp) | GenBank |
|---|---|---|---|---|
IL-1Rrp2 (A) | F: 5'-AGCAAAATCCCAGTGTCCAAA-3' | 60 | 291 | [AF284434] |
R: 5'-ACCCAAAACACAACTCTTCGG-3' | ||||
IL-1Rrp2 (B) | F: 5'-AGCAAAATCCCAGTGTCCAAA-3' | 60 | 147 | [AF284434] |
R: 5'-GGTTTACATGTATTCTATGACAG-3' | ||||
IL-1F8 | F: 5'-ACCAAGGAGAGAGGCATAACTAAT-3' | 60 | 147 | [NM173178] |
R: 5'-AGTGAACTCAGTCGCATAATGATC-3' | ||||
IL-1β | F: 5'-GCTGAGGAAGATGCTGGTTC-3' | 57 | 146 | [NM000756] |
R: 5'-GTGATCGTACAGGTGCATCG-3' | ||||
β-actin | F: 5'-CCAAGGCCAACCGCGAGAAGATGAC-3' | 55 | 579 | [M10277] |
R: 5'-AGGGTACATGGTGGTGCCGCCAGAC-3' | ||||
28S | F: 5'-TTGAAAATCCGCGGGAGA-3' | 54 | 100 | [U13369] |
R: 5'-ACATTGTTCCAACATGCCAG-3' |