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Table 1 Summary of primers used

From: The new IL-1 family member IL-1F8 stimulates production of inflammatory mediators by synovial fibroblasts and articular chondrocytes

cDNA Forward and reverse primers Ta (°C) Product (bp) GenBank
IL-1Rrp2 (A) F: 5'-AGCAAAATCCCAGTGTCCAAA-3' 60 291 [AF284434]
  R: 5'-ACCCAAAACACAACTCTTCGG-3'    
IL-1Rrp2 (B) F: 5'-AGCAAAATCCCAGTGTCCAAA-3' 60 147 [AF284434]
  R: 5'-GGTTTACATGTATTCTATGACAG-3'    
IL-1F8 F: 5'-ACCAAGGAGAGAGGCATAACTAAT-3' 60 147 [NM173178]
  R: 5'-AGTGAACTCAGTCGCATAATGATC-3'    
IL-1β F: 5'-GCTGAGGAAGATGCTGGTTC-3' 57 146 [NM000756]
  R: 5'-GTGATCGTACAGGTGCATCG-3'    
β-actin F: 5'-CCAAGGCCAACCGCGAGAAGATGAC-3' 55 579 [M10277]
  R: 5'-AGGGTACATGGTGGTGCCGCCAGAC-3'    
28S F: 5'-TTGAAAATCCGCGGGAGA-3' 54 100 [U13369]
  R: 5'-ACATTGTTCCAACATGCCAG-3'    
  1. Shown are the primer sequences, annealing temperatures (Ta), lengths of the corresponding PCR products, and GenBank accession numbers of the DNA sequences. F, forward; IL, interleukin; IL-1Rrp2, IL-1 receptor related protein 2; R, reverse; PCR, polymerase chain reaction.