Figure 1From: Cyclooxygenases and prostaglandin E2 receptors in growth plate chondrocytes in vitro and in situ – prostaglandin E2dependent proliferation of growth plate chondrocytesCollagen protein and mRNA expression in cultured rat growth plate chondrocytes. Isolated rat chondrocytes were cultured until confluency. (a) Protein expression for collagen I, II and X was studied in cultured chondrocytes with type-specific antibodies and using the alkaline-phosphatase-anti-alkaline-phosphatase method. Collagen type I was expressed in the majority of the cultured cells. Collagen II was strongly detected in chondrocytes of polygonal shape, representing more than 80% of the cultured cells. In cultured chondrocytes, no reactivity towards the collagen X antibody was observed. The antigens of the antibodies are indicated below the figures. (b) mRNA expression of the various collagen types. PCR analysis revealed expression of mRNA for collagen (Coll) I and collagen II and only marginal expression of collagen X mRNA.Back to article page