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Table 1 Primers used for RT-PCR

From: Cyclooxygenases and prostaglandin E2 receptors in growth plate chondrocytes in vitro and in situ – prostaglandin E2dependent proliferation of growth plate chondrocytes

mRNA

Sequence of primer

Product (bp)

Accession numbers

EP-1

5' -GCT GTA CGC CTC GCA TCG TGG-3'

5' -GTG TTT CGA GCA TCC CAT GTA TCT-3'

404

NCBI:D16338

EP-2

5' -GAA CGC TAC CTC TCC ATC GG-3'

5' -TGA TGG TCA TAA TGG-3'

415

NCBI:D50589

COX-1EP-3

5' -GTTTGGTCTG GCGTCTTAGA AC-3'

5' -CTTGGAACAG GACCTTCTGA GT-3'

5' -TTTGCCTCCGCCTTCGCCTG-3'

5' -AGCAGCAGATAAACC-3'

399,359

U03388 NCBI:D14869

COX-2EP-4

5' -AATGAGTACC GCAAA-3'

5' -ATCTAGTCTG GAGCGGGAGG-3'

5' -TGCTCATCTGCTCCATTCCGC-3'

5' -ATGCGAACCTGGAAG-5'

420,407

NM011198NCBI:D28860

Col ICOX-1

5' -TGGTGACAAG GGTGAGACAG-3'

5' -TGAGGCAGGA AGCTGAAGTC-3'

5' -GTTTGGTCTG GCGTCTTAGA AC-3'

5' -CTTGGAACAG GACCTTCTGA GT-3'

329,399

Z78279NCBI:U03388

Col IICOX-2

5' -CTCCAGGTGT GAAGGGTGAG-3'

5' -GAACCTTGAG CACCTTCAGG-3'

5' -AATGAGTACC GCAAA-3'

5' -ATCTAGTCTG GAGCGGGAGG-3'

261,420

NM012929NCBI:NM011198

Col XI

5' -TGCCTCTTGT CAGTGCTAAC C-3'

5' -GCGTGCCGTT CTTATACAGG-3'

5' -TGGTGACAAG GGTGAGACAG-3'

5' -TGAGGCAGGA AGCTGAAGTC-3'

248

329

AJ131848NCBI:Z78279

β-actinCol II

5' -CATCACCATT GGCAATGAGC G-3'

5' -CTAGAAGCAT TTGCGGTCGG AC-3'

5' -CTCCAGGTGT GAAGGGTGAG-3'

5' -GAACCTTGAG CACCTTCAGG-3'

403,261

NM031144NCBI:NM012929

Col X

5' -TGCCTCTTGT CAGTGCTAAC C-3'

5' -GCGTGCCGTT CTTATACAGG-3'

248

NCBI:AJ131848

β-actin

5' -CATCACCATT GGCAATGAGC G-3'

5' -CTAGAAGCAT TTGCGGTCGG AC-3'

403

NCBI:NM031144

  1. Col, collagen; COX, cyclooxygenase; EP, prostaglandin E receptor.