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Table 1 Primers used for RT-PCR

From: Cyclooxygenases and prostaglandin E2 receptors in growth plate chondrocytes in vitro and in situ – prostaglandin E2dependent proliferation of growth plate chondrocytes

mRNA Sequence of primer Product (bp) Accession numbers
EP-1 5' -GCT GTA CGC CTC GCA TCG TGG-3'
5' -GTG TTT CGA GCA TCC CAT GTA TCT-3'
404 NCBI:D16338
EP-2 5' -GAA CGC TAC CTC TCC ATC GG-3'
5' -TGA TGG TCA TAA TGG-3'
415 NCBI:D50589
COX-1EP-3 5' -GTTTGGTCTG GCGTCTTAGA AC-3'
5' -CTTGGAACAG GACCTTCTGA GT-3'
5' -TTTGCCTCCGCCTTCGCCTG-3'
5' -AGCAGCAGATAAACC-3'
399,359 U03388 NCBI:D14869
COX-2EP-4 5' -AATGAGTACC GCAAA-3'
5' -ATCTAGTCTG GAGCGGGAGG-3'
5' -TGCTCATCTGCTCCATTCCGC-3'
5' -ATGCGAACCTGGAAG-5'
420,407 NM011198NCBI:D28860
Col ICOX-1 5' -TGGTGACAAG GGTGAGACAG-3'
5' -TGAGGCAGGA AGCTGAAGTC-3'
5' -GTTTGGTCTG GCGTCTTAGA AC-3'
5' -CTTGGAACAG GACCTTCTGA GT-3'
329,399 Z78279NCBI:U03388
Col IICOX-2 5' -CTCCAGGTGT GAAGGGTGAG-3'
5' -GAACCTTGAG CACCTTCAGG-3'
5' -AATGAGTACC GCAAA-3'
5' -ATCTAGTCTG GAGCGGGAGG-3'
261,420 NM012929NCBI:NM011198
Col XI 5' -TGCCTCTTGT CAGTGCTAAC C-3'
5' -GCGTGCCGTT CTTATACAGG-3'
5' -TGGTGACAAG GGTGAGACAG-3'
5' -TGAGGCAGGA AGCTGAAGTC-3'
248
329
AJ131848NCBI:Z78279
β-actinCol II 5' -CATCACCATT GGCAATGAGC G-3'
5' -CTAGAAGCAT TTGCGGTCGG AC-3'
5' -CTCCAGGTGT GAAGGGTGAG-3'
5' -GAACCTTGAG CACCTTCAGG-3'
403,261 NM031144NCBI:NM012929
Col X 5' -TGCCTCTTGT CAGTGCTAAC C-3'
5' -GCGTGCCGTT CTTATACAGG-3'
248 NCBI:AJ131848
β-actin 5' -CATCACCATT GGCAATGAGC G-3'
5' -CTAGAAGCAT TTGCGGTCGG AC-3'
403 NCBI:NM031144
  1. Col, collagen; COX, cyclooxygenase; EP, prostaglandin E receptor.