Figure 3
From: Autoimmune targeting of key components of RNA interference
Human and mouse anti-Su sera recognize in vitro translated hAgo2 protein. (a) K562 cells were radiolabeled and extract was immunoprecipitated with human anti-Su serum (lane 2) and compared with the migration pattern of the in vitro synthesized hAgo2 product (lane 1). (b) Immunoprecipitation of in vitro translated hAgo2 product using human anti-GW182 serum (lane 3), human anti-Su sera (lanes 4 and 5), and mouse anti-Su serum (lane 7) compared with normal human serum (NHS) and pre-immune mouse serum (PIM) (lanes 2 and 6, respectively). Lane 1 represents the in vitro transcription and translation mix prior to immunoprecipitation.