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Figure 3 | Arthritis Research & Therapy

Figure 3

From: Human, viral or mutant human IL-10 expressed after local adenovirus-mediated gene transfer are equally effective in ameliorating disease pathology in a rabbit knee model of antigen-induced arthritis

Figure 3

Effect of human IL-10 (hIL-10), viral IL-10 (vIL-10), and mutant human IL-10 (mut.hIL-10) on cartilage matrix metabolism. Twenty-four hours after antigen-induced arthritis (AIA) induction, rabbit knees received either Ad.hIL-10, Ad.vIL-10, Ad.mut.hIL-10 or adenovirus expressing enhanced green fluorescent protein (Ad.eGFP). At days 3 and 7, the knees were lavaged, and on day 7 the animals sacrificed and their knee joints collected. (a) As a measure of proteoglycan breakdown, the glycosaminoglycans (GAGs) released into the lavage fluids, were measured spectrophotometrically. (b) To measure the rates of proteoglycan synthesis, pieces of articular cartilage from the femoral condyles were used, and their in vitro 35S042- incorporation into macromolecular material measured. All values are expressed as the mean ± standard error of the mean. Asterisks denote values that differ at p < 0.05 (Student's t test).

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Arthritis Research & Therapy

ISSN: 1478-6362

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