Figure 3

Confocal micrographs of dendritic cells and macrophages pulsed with type II collagen (CII). (a-c) Macrophages and (d-f) dendritic cells were incubated in the (a,b,d,e) presence or (c,f) absence of 200 μg/ml CII for 30 minutes, stained for CII expression and analyzed by confocal microscopy. Magnification ×630, and bars denote (a) 6.63 μm, (b) 8.09 μm, (c) 5.0 μm, (d) 4.27 μm, (e) 4.64 μm and (f) 4.0 μm. More than 50 cells were examined for each treatment.