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Figure 5 | Arthritis Research & Therapy

Figure 5

From: Inhibition of macropinocytosis blocks antigen presentation of type II collagen in vitro and in vivoin HLA-DR1 transgenic mice

Figure 5

The effect of inhibitors of uptake on the intracellular processing of type II collagen (CII) by macrophages. Macrophages from HLA-DR1-tg mice were pulsed with a dilution series of (a,b) CII or (c,d) synthetic peptides in the absence (closed squares) or presence of cytochalasin D (triangles), amiloride (closed circles), 5-(N,N-dimethyl)amiloride (DMA; diamonds), monodansylcadaverine (MDC; open circles) or filipin (open squares) in the doses shown in the legend to Figure 1 for 5 hours. After fixation, plates were assayed with the (a,c) T cell hybridoma HCII-9.2 specific for the glycosylated epitope CII259–273 or (b,d) T cell hybridoma HCII-9.1 specific for the non-glycosylated form of the same epitope. IL-2 production by T cell hybridomas was assayed as proliferation of cytotoxic T cell line-2 (CTLL-2) cells in the presence of 3H-thymidine, and the results are presented as mean counts per minute (cpm) ± standard deviation (SD). A representative of three experiments is shown and error bars denote SD.

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