Figure 6
The effect of inhibitors of uptake on the intracellular processing of type II collagen (CII) by dendritic cells. Dendritic cells from HLA-DR1-tg mice were pulsed with a dilution series of (a,b) CII or (c,d) synthetic peptides in the absence (closed squares) or presence of cytochalasin D (triangles), amiloride (closed circles), 5-(N,N-dimethyl)amiloride (DMA; diamonds), monodansylcadaverine (MDC; open circles) or filipin (open squares) in the doses shown in the legend to Figure 1 for 5 hours. After fixation, plates were assayed with the (a,c) T cell hybridoma HCII-9.2 specific for the glycosylated epitope CII259–273 or (b,d) T cell hybridoma HCII-9.1 specific for the non-glycosylated form of the same epitope. Other details are as in the legend to Figure 4.