Figure 7

The effect of inhibitors of uptake on APC phenotype. (a) Dendritic cells or (b) macrophages were pretreated for 5 hours with cytochalasin D (open bars), monodansylcadaverine (MDC; ladder-hatched bars), 5-(N,N-dimethyl)amiloride (DMA; hatched bars), amiloride (cross-hatched bars) or filipin (back-hatched bars) in the doses shown in the legend to Figure 1 before preparation for flow cytometry. Data for the expression of HLA-DR1, CD40, CD80 and CD86 are shown as mean fluorescent intensity. No significant differences were detected for all inhibitors compared with untreated cells in three independent experiments by paired two-tailed t test (P > 0.05).