Gene | Primer | Sequence | RT-PCR product (base pairs) | Annealing temperature | Cycles | Reference |
---|---|---|---|---|---|---|
GAPDH | up | CCACCCATGGCAAATTCCATGGCA | 600 | 60 | 25 | Stratagene |
down | TCTAGACGGCAGGTCAGGTCCACC | |||||
Actin | up | TGAAGTCTGACGTGGACATC | 254 | 60 | 25 | [7] |
down | ACTCGTCATACTCCTGCTTG | |||||
COL1A2 | up | AGACCCAAGGACTATGAAGT | 509 | 55 | 25 | [NCBI: NM_000089] |
down | ACATCATTAGAGCCCTGTAG | |||||
COL2A1 | up | CATCTGGTTTGGAGAAACCATC | 606 | 60 | 37 | [NCBI: J00116] |
down | GCCCAGTTCAGGTCTCTTAG | |||||
COL3A1 | up | GATGGGGTCAAATGAAGGTGA | 546 | 60 | 25 | [NCBI: NM_000090] |
down | GCAGATGGGCTAGGATTCAAA | |||||
COL10A1 | up | CCTCTTGTTAGTGCCAACCAG | 424 | 60 | 37 | [NCBI: X98568] |
down | GAGCCACTAGGAATCCTGAG | |||||
Aggrecan | up | ACTTCCGCTGGTCAGATGGA | 111 | 55 | 37 | [8] |
down | TCTCGTGCCAGATCATCACC | |||||
TGF-β1 | up | CAGAAATACAGCAACAATTCCTGG | 187 | 60 | 37 | Stratagene |
down | TTGCAGTGTGTTATCCGTGCTGTC | |||||
BMP-7 | up | TTTGGGGCCAAGTTTTTCTG | 410 | 60 | 37 | [9] |
down | ACAGGAACTTCCGGGTCAAT | |||||
BMP-2 | up | GGGAAAACAACCCGGAGATT | 503 | 60 | 37 | [NCBI: NM_001200] |
down | TTAAGGCGTTTCCGCTGTTT | |||||
FGF-2 | up | TACAACTTCAAGCAGAAGAG | 283 | 60 | 37 | [10] |
down | CAGCTCTTAGCAGACATTGG | |||||
VEGF | up | AAGTGGTCCCAGGCTGCA | 296 | 60 | 37 | [NCBI: AY047581] |
down | ATCTCTCCTATGTGCTGGCC | |||||
IL-1 | up | AAGCAGCCATGGCAGAAGTA | 482 | 60 | 37 | [NCBI: M15840] |
down | GAACACCACTTGTTGCTCCA | |||||
TNF- | up | GAGTGACAAGCCTGTAGCCCATGTTGTAGCA | 444 | 60 | 37 | Clontech |
down | GCAATGATCCCAAAGTAGACCTGCCCAGACT |