Cellular lysates of synovial fibroblasts cultured in lovastatin contain histone-bound DNA fragments. Cell lysates and corresponding culture supernatants were assayed by Cell Death Detection ELISAPLUS for the presence of histone-bound DNA fragments. (a) Synovial fibroblasts were cultured for 72 hours in the presence of 0, 3 or 10 μM lovastatin. The dark bars represent fragments present in the cell lysate indicative of apoptosis; the light bars represent fragments present in the supernatant. Shown is a representative of three different experiments. (b) Synovial fibroblasts were cultured for 72 hours in 10 μM lovastatin supplemented or not with 5 μM farnesylpyrophosphate (FPP) or 5 μM geranylgeranylpyrophosphate (GGPP).