The steady-state and activated Akt pathway is affected by lovastatin. Rheumatoid arthritis synovial fibroblasts (2 lines shown) were cultured in the presence of 3 μM lovastatin or DMSO (solvent control) for 48 hours. They were then treated with 2 ng/ml IL-1 or 10 ng/ml tumor necrosis factor-α (TNF-α) for 15 minutes. (a) Cellular lysates were prepared, subjected to 12% PAGE and analysed by immunoblotting for pAkt, pJNK, pERK and actin. D, dimethylsulfoxide (DMSO); 3L, 3 μM lovastatin; D, IL-1, cells cultured with DMSO then induced with IL-1; D, TNF, cells cultured with DMSO then induced with TNF-α; 3L, IL-1, cells cultured with 3 μM lovastatin then induced with IL-1; 3L, TNF, cells cultured with 3 μM lovastatin then induced with TNF-α (b-d) Bands were quantified by UN-SCAN-IT and corrected for loading by actin (b) pAkt, (c) pJNK, (d) pERK.