Figure 1

Inhibition of leukocyte interaction with the postcapillary endothelium by ALP in vivo. (a) Inhibition of leukocyte rolling along the postcapillary endothelium by antileukoproteinase in vivo. The results show the fraction of leukocytes rolling along the endothelium of postcapillary synovial venules (expressed as a percentage of all passing leukocytes) in anti-collagen II (CII) antibody-induced DBA1/J mice pretreated with either antileukoproteinase (ALP; 100 and 300 μg), human serum albumin (hSA, 300 μg) or NaCl in comparison with healthy mice (HC) that did not receive the antibody challenge. Intravital fluorescence microscopy of the knee joints was performed at 6 and 24 hours after the systemic transfer of anti-CII mAb. Results are shown as means and SEM for n = 8 animals per group; *p < 0.05. (b) Inhibition of leukocyte adherence to the postcapillary endothelium by ALP in vivo. The results show the number of leukocytes adherent to the endothelium of postcapillary synovial venules (expressed as cells per mm² endothelial surface) in anti-CII antibody-induced DBA1/J mice pretreated with either ALP (100 and 300 μg), hSA (300 μg) or NaCl in comparison with healthy mice (HC) that did not receive the antibody challenge. Intravital fluorescence microscopy of the knee joints was performed at 6 and 24 hours after the systemic transfer of anti-CII mAb. Results are shown as means and SEM for n = 8 animals per group; *p < 0.05.