Figure 7

Inhibition of IL-1β-induced NF-κB transcriptional activity in glEND.2 cells by antileukoproteinase. Effects of antileukoproteinase (ALP) on glEND.2 cells that had been transfected with an NF-κB-luciferase reporter gene construct were analysed separately. The determination of luminescence signals in the luciferase assay of the lysed cells permitted the quantification of the IL-1β-induced upregulation of transcriptional NF-êB-activity (expressed as a percentage of luminescence maximum). The stimulatory cytokine effect on NF-κB activation (+IL-1β) above the level of the unstimulated control (- IL-1β control) was significantly suppressed by ALP (*p < 0.05) in a concentration-dependent manner (+IL-1β ALP 8 μM and +IL-1β ALP 20 μM). Results are means and SEM for 16 independent experiments.