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Figure 4 | Arthritis Research & Therapy

Figure 4

From: Inhibitory effect of ribbon-type NF-κB decoy oligodeoxynucleotides on osteoclast induction and activity in vitro and in vivo

Figure 4

Expression of NFATc1 protein in osteoclast precursor cells. (a-d) Immunohistochemistry of NFATc1 protein with specific antibody in osteoclast precursor cells. Bone marrow macrophages were incubated with M-CSF/RANKL for 48 hours after incubation with ribbon-type scrambled decoy oligodeoxynucleotide (RSODN) (c) or ribbon-type NF-κB decoy oligodeoxynucleotide (RNODN) (d), or were untreated alone (b). (a) Without reaction with primary antibody. The expression of NFATc1 by immunofluorescence is shown in each upper panel. Nuclei stained with 4',6-diamidino-2-phenylindole are shown in each lower panel. Original magnification × 100. (e) Measurement of fluorescent area of osteoclast precursor cells. The areas of fluorescent cells in RSODN-treated and RNODN-treated groups are shown as percentages over that of the untreated group. (n = 5 per group; *p < 0.001 compared with the RSODN-treated group.)

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