Figure 4

Improved binding of anti-collagen type II antibodies to cartilage deficient in collagen type IX. Examples of immunohistochemical staining of the ankle from the hind paws of newborn mice, injected intraperitoneally with 100 μg of biotinylated C1 antibodies, as described in the Materials and methods section. Pictures show the tibia to the far left and the talus and calcaneus to the far right. (a) Section from a B10.Q mouse; (b) the same area from a C9T-BQ mouse except that the tibia is out of the picture. In this example the calcaneus was used for calculations. The pictures were developed and handled identically.