| Â | Â | Â | Arthritic/total | Â | Simple maximal | Extended maximal | Anti-CII antibody |
|---|
Experiment | Strain | Group | (n/n) | Day of onset | arthritic score | arthritic score | titer (μg/ml) |
|---|
CIAa | C9T-DQ | Homozygous | 5/11 | 60.6 ± 39.1 | 8.6 ± 2.7e | nd | 371.7 ± 359.3 |
CIAa | C9T-DQ | Heterozygous | 9/17 | 50.9 ± 8.2 | 5.4 ± 2.0e | nd | 532.1 ± 529.2 |
CIAa | C9T-DQ | Wild type | 10/17 | 55.6 ± 19.0 | 5.1 ± 2.9e | nd | 454.3 ± 489.0 |
CIAb | C9T-BQ | Homozygous | 6/9 | 62.8 ± 13.6 | 8.3 ± 2.0 | 29.9 ± 15.3e | nd |
CIAb | B10.Q | Wild type | 5/9 | 66.4 ± 17.3 | 6.0 ± 2.3 | 23.8 ± 8.2e | nd |
SIAc | C9T-DQ | Homozygous | 9/18 | 44.3 ± 18.4 | 3.1 ± 1.2f | nd | nd |
SIAc | C9T-DQ | Heterozygous | 19/26 | 43.7 ± 30.1 | 2.7 ± 1.6f | nd | nd |
SIAc | C9T-DQ | Wild type | 12/17 | 39.2 ± 23.8 | 2.0 ± 0.9f | nd | nd |
CAIAd | C9T-BQ | Homozygous Ab | 13/20 | 5.5 ± 3.0g | 4.8 ± 2.8 | 12.8 ± 11.2 | nd |
CAIAd | C9T-BQ | Homozygous PBS | 0/8 | - | - | - | nd |
CAIAd | B10.Q | Wild-type Ab | 5/8 | 8.4 ± 0.5g | 5.6 ± 3.0 | 11.2 ± 8.5 | nd |
- Where errors are shown, results are means ± SD. aOne generation intercrossed C9T-DQ and littermate control females were immunized with 50 μg of CII. bPure lines of C9T-BQ and B10.Q male mice were immunized with 100 μg of CII. cOne generation intercrossed male C9T-DQ and littermate controls were randomly and pooled blind. dAnti-CII antibody-induced arthritis on pure line of male C9T-BQ (homozygous Ab) and male B10.Q (wild-type Ab), and controls injected with PBS (homozygous PBS). eHomozygous mice were significantly higher in score (p < 0.05) than both heterozygous (if present) and wild type, respectively. There were no other significant differences between the groups within the experiments. fHomozygous mice were significantly higher in score (p = 0.025) than wild type but not heterozygous. There were no other significant differences between the groups. gHomozygous mice that received Ab had a significantly earlier onset (p = 0.035) with six arthritic mice before lipopolysaccharide injection, than their wild-type counterpart. Two homozygous mice injected with Ab also developed knee inflammation at days 4 and 6, which were not seen in wild-type Ab. CAIA, collagen-antibody-induced arthritis; CIA, collagen-induced arthritis; CII, type II collagen; nd, not determined; SIA = stress-induced arthritis.
