| | | |
Arthritic/total
| |
Simple maximal
|
Extended maximal
|
Anti-CII antibody
|
|---|
|
Experiment
|
Strain
|
Group
|
(n/n)
|
Day of onset
|
arthritic score
|
arthritic score
|
titer (μg/ml)
|
|---|
|
CIAa
|
C9T-DQ
|
Homozygous
|
5/11
|
60.6 ± 39.1
|
8.6 ± 2.7e
|
nd
|
371.7 ± 359.3
|
|
CIAa
|
C9T-DQ
|
Heterozygous
|
9/17
|
50.9 ± 8.2
|
5.4 ± 2.0e
|
nd
|
532.1 ± 529.2
|
|
CIAa
|
C9T-DQ
|
Wild type
|
10/17
|
55.6 ± 19.0
|
5.1 ± 2.9e
|
nd
|
454.3 ± 489.0
|
|
CIAb
|
C9T-BQ
|
Homozygous
|
6/9
|
62.8 ± 13.6
|
8.3 ± 2.0
|
29.9 ± 15.3e
|
nd
|
|
CIAb
|
B10.Q
|
Wild type
|
5/9
|
66.4 ± 17.3
|
6.0 ± 2.3
|
23.8 ± 8.2e
|
nd
|
|
SIAc
|
C9T-DQ
|
Homozygous
|
9/18
|
44.3 ± 18.4
|
3.1 ± 1.2f
|
nd
|
nd
|
|
SIAc
|
C9T-DQ
|
Heterozygous
|
19/26
|
43.7 ± 30.1
|
2.7 ± 1.6f
|
nd
|
nd
|
|
SIAc
|
C9T-DQ
|
Wild type
|
12/17
|
39.2 ± 23.8
|
2.0 ± 0.9f
|
nd
|
nd
|
|
CAIAd
|
C9T-BQ
|
Homozygous Ab
|
13/20
|
5.5 ± 3.0g
|
4.8 ± 2.8
|
12.8 ± 11.2
|
nd
|
|
CAIAd
|
C9T-BQ
|
Homozygous PBS
|
0/8
|
-
|
-
|
-
|
nd
|
|
CAIAd
|
B10.Q
|
Wild-type Ab
|
5/8
|
8.4 ± 0.5g
|
5.6 ± 3.0
|
11.2 ± 8.5
|
nd
|
- Where errors are shown, results are means ± SD. aOne generation intercrossed C9T-DQ and littermate control females were immunized with 50 μg of CII. bPure lines of C9T-BQ and B10.Q male mice were immunized with 100 μg of CII. cOne generation intercrossed male C9T-DQ and littermate controls were randomly and pooled blind. dAnti-CII antibody-induced arthritis on pure line of male C9T-BQ (homozygous Ab) and male B10.Q (wild-type Ab), and controls injected with PBS (homozygous PBS). eHomozygous mice were significantly higher in score (p < 0.05) than both heterozygous (if present) and wild type, respectively. There were no other significant differences between the groups within the experiments. fHomozygous mice were significantly higher in score (p = 0.025) than wild type but not heterozygous. There were no other significant differences between the groups. gHomozygous mice that received Ab had a significantly earlier onset (p = 0.035) with six arthritic mice before lipopolysaccharide injection, than their wild-type counterpart. Two homozygous mice injected with Ab also developed knee inflammation at days 4 and 6, which were not seen in wild-type Ab. CAIA, collagen-antibody-induced arthritis; CIA, collagen-induced arthritis; CII, type II collagen; nd, not determined; SIA = stress-induced arthritis.
