Skip to main content

Advertisement

Table 1 Summary of experiments conducted on C9T mice

From: Type IX collagen deficiency enhances the binding of cartilage-specific antibodies and arthritis severity

    Arthritic/total   Simple maximal Extended maximal Anti-CII antibody
Experiment Strain Group (n/n) Day of onset arthritic score arthritic score titer (μg/ml)
CIAa C9T-DQ Homozygous 5/11 60.6 ± 39.1 8.6 ± 2.7e nd 371.7 ± 359.3
CIAa C9T-DQ Heterozygous 9/17 50.9 ± 8.2 5.4 ± 2.0e nd 532.1 ± 529.2
CIAa C9T-DQ Wild type 10/17 55.6 ± 19.0 5.1 ± 2.9e nd 454.3 ± 489.0
CIAb C9T-BQ Homozygous 6/9 62.8 ± 13.6 8.3 ± 2.0 29.9 ± 15.3e nd
CIAb B10.Q Wild type 5/9 66.4 ± 17.3 6.0 ± 2.3 23.8 ± 8.2e nd
SIAc C9T-DQ Homozygous 9/18 44.3 ± 18.4 3.1 ± 1.2f nd nd
SIAc C9T-DQ Heterozygous 19/26 43.7 ± 30.1 2.7 ± 1.6f nd nd
SIAc C9T-DQ Wild type 12/17 39.2 ± 23.8 2.0 ± 0.9f nd nd
CAIAd C9T-BQ Homozygous Ab 13/20 5.5 ± 3.0g 4.8 ± 2.8 12.8 ± 11.2 nd
CAIAd C9T-BQ Homozygous PBS 0/8 - - - nd
CAIAd B10.Q Wild-type Ab 5/8 8.4 ± 0.5g 5.6 ± 3.0 11.2 ± 8.5 nd
  1. Where errors are shown, results are means ± SD. aOne generation intercrossed C9T-DQ and littermate control females were immunized with 50 μg of CII. bPure lines of C9T-BQ and B10.Q male mice were immunized with 100 μg of CII. cOne generation intercrossed male C9T-DQ and littermate controls were randomly and pooled blind. dAnti-CII antibody-induced arthritis on pure line of male C9T-BQ (homozygous Ab) and male B10.Q (wild-type Ab), and controls injected with PBS (homozygous PBS). eHomozygous mice were significantly higher in score (p < 0.05) than both heterozygous (if present) and wild type, respectively. There were no other significant differences between the groups within the experiments. fHomozygous mice were significantly higher in score (p = 0.025) than wild type but not heterozygous. There were no other significant differences between the groups. gHomozygous mice that received Ab had a significantly earlier onset (p = 0.035) with six arthritic mice before lipopolysaccharide injection, than their wild-type counterpart. Two homozygous mice injected with Ab also developed knee inflammation at days 4 and 6, which were not seen in wild-type Ab. CAIA, collagen-antibody-induced arthritis; CIA, collagen-induced arthritis; CII, type II collagen; nd, not determined; SIA = stress-induced arthritis.