Table 1 Probes and primer sequences used
Gene | Primers and probes |
|---|---|
11β-HSD1 | Forward primer: AGGAAAGCTCATGGGAGGACTAG |
Reverse primer: ATGGTGAATATCATCATGAAAAAGATTC | |
Probe: CATGCTCATTCTCAACCACATCACCAACA | |
H6PDH | Forward primer: CAGGTGTCCTAGTGCACATTGAC |
Reverse primer: GTAGCCCACTCTCTCGTCCAA | |
Probe: AAGGCACGCCCTCCCAGCG | |
GRα | Forward primer: GCGATGGTCTCAGAAACCAAAC |
Reverse primer: GAGATTACAGAGGAAGTTATCCTCTGC | |
Probe: TGCAGTGAAGGTTGCTGAGGCTCTGA | |
GRβ | Forward primer: AAC TGG CAG CGG TTT TAT CAA CT |
Reverse primer: AACTCTTGGATTCTATGCATGAAAATGTTA TGTGGTTA | |
Probe: TGT GTG AGA TGT GCT TTC TGG TT | |
C/EBPα | Forward primer: TGGACAAGAACAGCAACGAG |
Reverse primer: TTGTCACTGGTCAGCTCCAG | |
Probe: CACCTTCTGCTGCGTCTCCACGTT | |
C/EBPβ | Forward primer: GACAAGCACAGCGACGAGTA |
Reverse primer: GTGCTGCGTCTCCAGGTT | |
Probe: ATCTTGGCCTTGTCGCGGCTCTT | |
IL-6 | Assay on demand Hs00174131_m1 (ABI) |