A cell-cycle independent role for p21 in regulating synovial fibroblast migration in rheumatoid arthritis

Table 1 Migratory differences between wild-type and p21^(-/-) fibroblast-like synoviocytes are due to altered chemotaxis and chemokinesis

	RA SF dilution across membrane
RA SF dilution in lower wells	None	1:100	1:75	1:50
None	3 ± 0.9	142 ± 9	156 ± 11	194 ± 9
1:100	16 ± 3	133 ± 8	122 ± 5	185 ± 13
1:75	18 ± 2	92 ± 3	143 ± 7	209 ± 7
1:50	12 ± 2	116 ± 8	100 ± 4	130 ± 9

Checkerboard assay results of p21^(-/-) fibroblast-like synoviocytes (FLS) migration are presented such that dilutions of rheumatoid arthritis (RA) synovial fluids (SFs), which were included with the cells (in the lower wells), are displayed down the left hand column. Dilutions of RA SFs across the membrane from the cells are displayed in the top row. Numbers represent the sum of counts from three high power fields determined from quadruplicate wells ± standard error (SE). These data are representative of three independent checkerboard assays.

ISSN: 1478-6362