Figure 3
Effect of hepatocyte growth factor (HGF) on host B cell activation and donor anti-host cytotoxic T lymphocyte (CTL) activity. Chronic graft-versus-host disease (GVHD) mice were treated as described in Figure 1. (a) At 2 weeks after GVHD induction, spleens were removed and H-2Kb+B220+ cells examined by flow cytometry. Data represent mean ± SD for four mice per group. (b) At 2 weeks after GVHD induction, serum IgG1 concentrations were determined by ELISA. Data represent mean ± SD for four mice per group. (c) At 2 weeks after GVHD induction, serum anti-single-stranded DNA antibody concentrations were determined by ELISA. Data represent mean ± SD for four mice per group. (d) At 2 weeks after GVHD induction, spleen cells were stimulated with irradiated BDF1 spleen cells for 5 days. Cytotoxicity was determined by CTL activity against 51Cr-labeled EL-4 (H-2b) target cells. CTL activity against P815 cells (H-2d) by acute GVHD mouse spleen cells was used as a positive control. Effector cells were tested in triplicate at four effector:target (E/T) ratios and the percent lysis calculated. Data represent mean ± SD for four mice per group.