Figure 6
In vitro effect of hepatocyte growth factor (HGF) on T helper (Th)2 generation from DBA/2 CD4+ T cells. CD4+ T cells from DBA/2 (H-2d) mice (4 × 106/ml/well) were cultured with irradiated (20 Gy) CD11c+ dendritic cell (DCs) from BDF1 (H-2b×d) mice (1 × 106/ml/well) in the presence or absence of human recombinant HGF (10 ng/ml). CD4+ T cells from DBA/2 (H-2d) mice (4 × 106/ml/well) cultured without CD11c+ DC stimulation were used as controls. After 72 h, viable cells (1 × 105/200 μl/well) were harvested and stimulated with anti-CD3 mAb (5 μg/ml) for 48 h. The concentration of IL-4 (a) and IFN-γ (b) in the culture supernatant was measured by ELISA. Data represent mean ± SD of three independent experiments. *p < 0.05; **p < 0.01.