Figure 7
Effect of hepatocyte growth factor (HGF) on CD4+ T cell CD28 expression and on CD11c+ dendritic cell (DC) CD86 expression. (a) CD4+ T cells from DBA/2 (H-2d) or B6 (H-2b) mice (4 × 106/ml/well) were cultured in the presence or absence of human recombinant HGF (10 ng/ml). After 48 h, mean fluorescence intensity of CD28 expression on CD4+ T cells determined. Data represent mean ± SD of three independent experiments. *p < 0.05. (b,c) CD4+ T cells from DBA/2 (H-2d) or B6 (H-2b) mice (4 × 106/ml/well) were cultured with irradiated (20 Gy) CD11c+ DCs from BDF1 (H-2b×d) mice (1 × 106/ml/well) in the presence or absence of human recombinant HGF (10 ng/ml) for 48 h, and mean fluorescence intensity of CD28 expression on CD4+ T cells (b) and CD86 expression on CD11c+ DCs (c) determined. Data represent mean ± SD of three independent experiments. *p < 0.05; **p < 0.01.