Figure 2
Induction of monocyte expression of type 1 and type 2 interleukin-10 receptor (IL-10R1/2) by culture supernatants of rheumatoid arthritis (RA) synovial tissue (ST) cells. Purified normal monocytes from different individuals (5 × 105 cells in culture medium with 10% fetal calf serum) were incubated for 3 days with or without a 20% concentration of RA ST cell culture supernatants, and IL-10R1 and IL-10R2 expression was analysed by flow cytometric analysis with anti-IL-10R1, anti-IL-10R2, and control antibodies. The induction of monocyte IL-10R1 and IL-10R2 expression by supernatants was expressed by the ratio of the mean fluorescence intensity (MFI) of IL-10R expression with supernatants to the MFI without supernatants. Values are the mean ± standard error of the mean. n, number of samples tested.